microBrain 3D spheroids exhibit synchronized and spontaneous oscillations.
Each well of the microBrain 3D platform contains a single, uniform neurospheroid comprised of cortical neurons and astrocytes making it ideal for high-throughput drug and toxicity screening studies.
microBrain 3D spheroids are comprised of a physiologic mixture of neurons (MAP2) and astrocytes (GFAP). Nuclei in blue
When stimulated with GABA , microBrain 2D
exhibits appropriate and spontaneous neural firing in response to neurostimulatory chemicals
microBrain 2D
​
HuC/D (green) (cell bodies)
GABA (red) (GABAergic neurons)
NF-M (pink) (neuronal filaments in neurons)
​
20x magnification
microBrain
2D and 3D Physiologically-Relevant Human iPSC-derived High-Throughput Screening Platforms
microBrain 2D
​
S100B (green) (immature astrocytes)
GFAP (red) (mature astrocytes)
MAP2 (pink) (neuronal filament marker in neurons)
​
5X Magnification
The power and effectiveness of the microBrain Assay Ready platforms' enhanced network structure and cell maturity are evident through highly synchronized activity. Containing a physiologic mixture of neurons and astrocytes co-matured from a single donor source, cells in the StemoniX microBrain platforms express key markers of cell maturity and a high density of key neural synapses.
​
microBrain 3D cortical spheroids present quantifiable, robust and uniform spontaneous and synchronized calcium oscillations that can be measured in kinetic high-throughput plate readers (e.g. FLIPR) and high content imaging systems.
​
microBrain 2D cultures, also high-throughput, are ideal for imaging and infection studies.
​
microBrain calcium signals can be reproducibly modified with excitatory and inhibitory modulators, confirming the presence of fully functional and integrated glutamatergic/GABAergic circuitry. The StemoniX microBrain platforms demonstrate remarkably consistent performance, making them ideal for high throughput drug discovery, imaging, and screening applications.
​
Both platforms can be customized to suit your discovery needs.