Functional Testing

Using biologically relevant human neural cell-based assays in drug discovery mimics the complexity of the human brain and improves predictivity of in vitro drug screening assays. 

 

The StemoniX microBrain 3D platform is spontaneously active and can be used to model both the native neurological conditions as well as neurological disorders to ascertain compound impact in high-throughput.

 

Our screening experts can thoroughly test your compounds to de-risk your development process.

General Procedure:

  1. Cell model is plated and maintained in the 384-well format plate until ready for assay.

  2. Plates are treated with test compounds for indicated time periods.

  3. After the desired compound incubation is completed, cells are treated with Calcium 6 dye (Molecular Devices), and the plate is read using the FLIPR Tetra.
        Ten-minute reads can be performed at a range of intervals from 0-6 hours post-treatment.

  4. Data are exported and analyzed to provide dose response curves and EC50s for each compound.

  5. Two key parameters (peak count and peak amplitude) are provided as part of the standard data package, but up to 10 parameters can be analyzed as a custom data analysis package.

Need more than functional data? Pair this assay with a cell viability assay using CellTiter Glo or LDH-Glo, or collect cell pellets post-FLIPR assay for gene expression studies.
WS_MB 3D plate BF.png
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microBrain 3D neurospheroids (back) show varying patterns of calcium oscillations in response to compound treatment (front).

Control microBrain spheroid showing synchronized and spontaneous oscillations

microBrain spheroid showing rapid and erratic oscillations in response to treatment with 4-AP

microBrain 3D neurospheroids (back) show varying patterns of calcium oscillations in response to compound treatment (front).