microBrain 3D spheroids exhibit synchronized and spontaneous oscillations.

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Each well of  the microBrain 3D platform contains a single, uniform neurospheroid comprised of cortical neurons and astrocytes making it ideal for high-throughput drug and toxicity screening studies.

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microBrain 3D spheroids are comprised of a physiologic mixture of neurons (MAP2) and astrocytes (GFAP). Nuclei in blue

When stimulated with GABA , microBrain 2D

exhibits appropriate and spontaneous neural firing in response to neurostimulatory chemicals

HUCD, GABA, and MAP2 staining of microBrain 2D
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microBrain 2D

HuC/D (green) (cell bodies)

GABA (red) (GABAergic neurons)

NF-M (pink) (neuronal filaments in neurons)

20x magnification

Mature neurons and astrocytes in microBrain 2D showing synapses
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microBrain 2D

S100B (green) (immature astrocytes)

GFAP (red) (mature astrocytes)

MAP2 (pink) (neuronal filament marker in neurons)

5X Magnification

microBrain

2D and 3D Physiologically-Relevant Human iPSC-derived High-Throughput Screening Platforms

The power and effectiveness of the microBrain Assay Ready platforms' enhanced network structure and cell maturity are evident through highly synchronized activity. Containing a physiologic mixture of neurons and astrocytes co-matured from a single donor source, cells in the StemoniX microBrain platforms express key markers of cell maturity and a high density of key neural synapses. 

microBrain 3D cortical spheroids present quantifiable, robust and uniform spontaneous and synchronized calcium oscillations that can be measured in kinetic high-throughput plate readers (e.g. FLIPR) and high content imaging systems.

 

microBrain 2D cultures, also high-throughput, are ideal for imaging and infection studies. 

microBrain calcium signals can be reproducibly modified with excitatory and inhibitory modulators, confirming the presence of fully functional and integrated glutamatergic/GABAergic circuitry. The StemoniX microBrain platforms demonstrate remarkably consistent performance, making them ideal for high throughput drug discovery, imaging, and screening applications.

Both platforms can be customized to suit your discovery needs.

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