Viability Assays

Using biologically relevant human neural cell-based assays in drug discovery mimics the complexity of the human brain and improves predictivity of in vitro drug screening assays. 

 

With a physiologically-relevant mixture of cortical neurons and astroycytes, the StemoniX microBrain platform is perfect for both imaging and metabolic toxicity testing. 

 

CellTiter-GLO

General Procedure:

  1. Cell model will be plated and maintained in the 384-well format plate until ready for assay.

  2. Plates are treated with test articles

  3. After the desired compound incubation is completed, run the cell viability assay using the Promega Cell-Titer-Glo assay kit.  

  4. Data is exported and analyzed to provide dose response curves and EC50s for each
    test article.

CTG signal over time generating using the Cell-Titer Glo 3D assay from Promega.

Need data collected over time, rather than a single timepoint? Check out our cell viability assay using LDH-Glo, which can be performed at multiple timepoints throughout the dosing period!

LDH-GLO

General Procedure:

  1. Cell model will be plated and maintained in the 384-well format plate until ready for assay.

  2. Plates are treated with test articles

  3. Cell supernatant will be collected from each well at the desired timepoints during or after test article treatment.

  4. Prepare LDH standard curve and run assay using the Promega LDH-Glo kit.

  5. Data is exported and analyzed to provide dose response curves and EC50s for each test article.

 

Prefer a high content screening approach to viability? Check out our cell viability assays using Calcein AM.

 

Calcein AM High Content Screening

General Procedure:

  1. Cell model will be plated and maintained in the 384-well format plate until ready for assay.

  2. Plates are treated with test articles

  3. Cells are fixed using 4% PFA

  4. High content imaging is performed using the Perkin Elmer Operetta CLS

  5. Data are analyzed to provide % live cells, average Calcein AM intensity by condition, and average nuclear stain intensity

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*Significant changes can be observed in nuclear intensity following test article treatment. This can indicate signs of nuclear condensation, apoptosis or pyknosis.